![]() ![]() It passes through the field iris diaphragm. The light source is housed in the base of the microscope. What does the field iris diaphragm control? Make sure you re-adjust the iris diaphragm for each objective lens. If the diaphragm is closed, the image is darker but the contrast greater. If the diaphragm is open, the image is brighter but the contrast is low. Why is adjusting iris diaphragm important?Īdjust the iris diaphragm to achieve the optimum balance between definition and glare. Closing the iris diaphragm will reduce the amount of illumination of the specimen but increases the amount of contrast. In light microscopy the iris diaphragm controls the size of the opening between the specimen and condenser, through which light passes. What happens when you move the iris diaphragm of the microscope? 6 How many blades are in an iris diaphragm?.5 How is the field diaphragm used in Zeiss microscopy?.4 How does the iris diaphragm affect resolution?.3 When should the iris diaphragm be used?.2 Why is adjusting iris diaphragm important?.1 What happens when you move the iris diaphragm of the microscope?.
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